NIH—NATIONAL LIBRARY OF MEDICINENATIONAL CENTER FOR BIOTECHNOLOGY INFORMATION



Ozone therapy: A clinical review.

Elvis AM, Ekta JS.J Nat Sci Biol Med. 2011 Jan;2(1):66-70. doi: 10.4103/0976-9668.82319.PMID: 22470237 

 

Emerging roles of ozone in skin diseases.

PMID: 23717326 

 

Oxygen-ozone autohemotherapy against COVID-19 needs to fit highly experienced, customized, and standardized protocols to succeed.

Chirumbolo S, Franzini M, Simonetti V, Valdenassi L, Ricevuti G, Bertossi D, Pandolfi S.J Med Virol. 2021 Jan 27. doi: 10.1002/jmv.26806. Online ahead of print.PMID: 33503267 

 

 

Molecular insights into the effect of ozone on human hemoglobin in autohemotherapy: Highlighting the importance of the presence of blood antioxidants during ozonation.

Mehraban F, Seyedarabi A, Seraj Z, Ahmadian S, Poursasan N, Rayati S, Moosavi-Movahedi AA.Int J Biol Macromol. 2018 Nov;119:1276-1285. doi: 10.1016/j.ijbiomac.2018.08.028. Epub 2018 Aug 8.PMID: 30096397

 

erman.

The influence of ozonated autohemotherapy on oxidative stress in hemodialyzed patients with atherosclerotic ischemia of lower limbs.

Tylicki L, Nieweglowski T, Biedunkiewicz B, Chamienia A, Debska-Slizien A, Aleksandrowicz E, Lysiak-Szydlowska W, Rutkowski B.Int J Artif Organs. 2003 Apr;26(4):297-303. doi: 10.1177/039139880302600404.PMID: 12757028 Clinical Trial.

 

Estrogenic activity removal of 17beta-estradiol by ozonation and identification of by-products.

Bila D, Montalvão AF, Azevedo Dde A, Dezotti M.Chemosphere. 2007 Oct;69(5):736-46. doi: 10.1016/j.chemosphere.2007.05.016. Epub 2007 Jun 22.PMID: 17588638

 

Time and time-frequency analysis of near-infrared signals for the assessment of ozone autohemotherapy long-term effects in multiple sclerosis.

Lintas G, Molinari F, Simonetti V, Franzini M, Liboni W.Annu Int Conf IEEE Eng Med Biol Soc. 2013;2013:6171-4. doi: 10.1109/EMBC.2013.6610962.PMID: 24111149

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Studies on the biological effects of ozone: 7. Generation of reactive oxygen species (ROS) after exposure of human blood to ozone.

Bocci V, Valacchi G, Corradeschi F, Aldinucci C, Silvestri S, Paccagnini E, Gerli R.J Biol Regul Homeost Agents. 1998 Jul-Sep;12(3):67-75.PMID: 9795834

 

Quantification of the ozone and singlet delta oxygen produced in gas and liquid phases by a non-thermal atmospheric plasma with relevance for medical treatment.

Jablonowski H, Santos Sousa J, Weltmann KD, Wende K, Reuter S.Sci Rep. 2018 Aug 15;8(1):12195. doi: 10.1038/s41598-018-30483-w.PMID: 30111826 

 

Similarities and differences of hyperbaric oxygen and medical ozone applications.

Ozler M, Akay C, Oter S, Ay H, Korkmaz A.Free Radic Res. 2011 Nov;45(11-12):1267-78. doi: 10.3109/10715762.2011.627331. Epub 2011 Oct 24.PMID: 21955297 Review.

 

Ozone: a new therapeutic agent in vascular diseases.

Bocci V, Zanardi I, Travagli V.Am J Cardiovasc Drugs. 2011;11(2):73-82. doi: 10.2165/11539890-000000000-00000.PMID: 21446774 Review

 

Toxicity of polycyclic aromatic hydrocarbons. I. Effect of phenanthrene, pyrene, and their ozonized products on blood chemistry in rats.

Yoshikawa T, Ruhr LP, Flory W, Giamalva D, Church DF, Pryor WA.Toxicol Appl Pharmacol. 1985 Jun 30;79(2):218-26. doi: 10.1016/0041-008x(85)90343-6.PMID: 2860738

 

Treatment with ozone/oxygen-pneumoperitoneum results in complete remission of rabbit squamous cell carcinomas.

Schulz S, Häussler U, Mandic R, Heverhagen JT, Neubauer A, Dünne AA, Werner JA, Weihe E, Bette M.Int J Cancer. 2008 May 15;122(10):2360-7. doi: 10.1002/ijc.23382.PMID: 18224691 Clinical Trial

28;43(2):114-123. doi: 10.11817/j.issn.1672-7347.2018.02.002.PMID: 29559592 

 

Validity of Oxygen-Ozone Therapy as Integrated Medication Form in Chronic Inflammatory Diseases.

Bocci V, Zanardia I, Valacchi G, Borrelli E, Travagli V. Cardiovasc Hematol Disord Drug Targets. 2015;15(2):127-38. doi: 10.2174/1871529×1502151209114642.PMID: 26126818 Review.


 

Inactivation of fungi associated with barley grain by gaseous ozone.

Allen B, Wu J, Doan H.J Environ Sci Health B. 2003 Sep;38(5):617-30. doi: 10.1081/PFC-120023519.PMID: 12929720

 

 

[Precise application of Traditional Chinese Medicine in minimally-invasive techniques].

Dong FH.Zhongguo Gu Shang. 2018 Jun 25;31(6):493-496. doi: 10.3969/j.issn.1003-0034.2018.06.001.PMID: 29945400 Chinese.

 

Anton syndrome during oxygen-ozone therapy.

Avcı S, Büyükcam F, Demir ÖF, Özkan S.Am J Emerg Med. 2015 Jun;33(6):856.e1-2. doi: 10.1016/j.ajem.2014.11.041. Epub 2014 Dec 2.PMID: 25511367

 

Ozone therapy: an overview of pharmacodynamics, current research, and clinical utility.

Smith NL, Wilson AL, Gandhi J, Vatsia S, Khan SA.Med Gas Res. 2017 Oct 17;7(3):212-219. doi: 10.4103/2045-9912.215752. eCollection 2017 Jul-Sep.PMID: 29152215 




Personalizing the safe, appropriate and effective concentration(s) of ozone for a non-diabetic individual and four type II diabetic patients in autohemotherapy through blood hemoglobin analysis


Fouad Mehraban  1 Arefeh Seyedarabi  2 Shahin Ahmadian  1 Vahid Mirzaaghaei  3 Ali Akbar Moosavi-Movahedi  1

Affiliations expand

PMID: 31311548

 PMCID:  PMC6636166

 DOI:  10.1186/s12967-019-1973-5

Abstract

Background: Diabetes is a chronic disease associated with many problems and high costs. In recent decades, a lot of research has been carried out in order to improve methods of treatment of diabetic patients. One of the currently used complementary therapies for diabetes is ozone therapy or autohemotherapy. The beneficial effects of ozone has been proven in many diseases such as diabetes, but the critical issue is the determination of the safe and effective concentration of ozone reacting with blood and in particular hemoglobin.

Methods: A number of spectroscopic techniques including intrinsic fluorescence, circular dichroism and UV-VIS spectroscopies were used as well as SDS-PAGE, Native-PAGE and dynamic light scattering to analyze the effect of ozonation on hemoglobin of a non-diabetic individual and four diabetic patients in order to find the appropriate concentration(s) of ozone for personalized autohemotherapy.

Results: In this study, we determined the personalized concentration(s) for a safe and effective ozonation of a non-diabetic individual and four diabetic type II patients, based on blood hemoglobin analysis.

Cited by 3  articles

Antimycotic Activity of Ozonized Oil in Liposome Eye Drops against Candida spp.Celenza G, Iorio R, Cracchiolo S, Petricca S, Costagliola C, Cinque B, Segatore B, Amicosante G, Bellio P. Transl Vis Sci Technol. 2020 Jul 2;9(8):4. doi: 10.1167/tvst.9.8.4. eCollection 2020 Jul.PMID: 32855851 .

Ozonated Oils as Antimicrobial Systems in Topical Applications. Their Characterization, Current Applications, and Advances in Improved Delivery Techniques.Ugazio E, Tullio V, Binello A, Tagliapietra S, Dosio F. Molecules. 2020 Jan 14;25(2):334. doi: 10.3390/molecules25020334.PMID: 31947580 

  The antibacterial effect of topical ozone on the treatment of MRSA skin infection.

Song M, Zeng Q, Xiang Y, Gao L, Huang J, Huang J, Wu K, Lu J.Mol Med Rep. 2018 Feb;17(2):2449-2455. doi: 10.3892/mmr.2017.8148. Epub 2017 Nov 24.PMID: 29207120  F



Effect of low-dose gaseous ozone on pathogenic bacteria




Belchor Fontes  1 Ana Maria Cattani Heimbecker Glacus de Souza Brito Silvia F Costa Inneke M van der Heijden Anna S Levin Samir Rasslan

Affiliations expand

·       PMID: 23249441

·       PMCID:  PMC3541223

·       DOI:  10.1186/1471-2334-12-358


Abstract

Background: Treatment of chronically infected wounds is a challenge, and bacterial environmental contamination is a growing issue in infection control. Ozone may have a role in these situations. The objective of this study was to determine whether a low dose of gaseous ozone/oxygen mixture eliminates pathogenic bacteria cultivated in Petri dishes.

Methods: A pilot study with 6 bacterial strains was made using different concentrations of ozone in an ozone-oxygen mixture to determine a minimally effective dose that completely eliminated bacterial growth. The small and apparently bactericidal gaseous dose of 20 μg/mL ozone/oxygen (1:99) mixture, applied for 5 min under atmospheric pressure was selected. In the 2nd phase, eight bacterial strains with well characterized resistance patterns were evaluated in vitro using agar-blood in adapted Petri dishes (105 bacteria/dish). The cultures were divided into 3 groups: 1–ozone-oxygen gaseous mixture containing 20 μg of O(3)/mL for 5 min; 2–100% oxygen for 5 min; 3–baseline: no gas was used.

Results: The selected ozone dose was applied to the following eight strains: Escherichia coli, oxacillin-resistant Staphylococcus aureus, oxacillin-susceptible Staphylococcus aureus, vancomycin-resistant Enterococcus faecalis, extended-spectrum beta-lactamase-producing Klebsiella pneumoniae, carbapenem-resistant Acinetobacter baumannii, Acinetobacter baumannii susceptible only to carbapenems, and Pseudomonas aeruginosa susceptible to imipenem and meropenem. All isolates were completely inhibited by the ozone-oxygen mixture while growth occurred in the other 2 groups.

Conclusion: A single topical application by nebulization of a low ozone dose completely inhibited the growth of all potentially pathogenic bacterial strains with known resistance to antimicrobial agents.



Therapeutic relevance of ozone therapy in degenerative diseases: Focus on diabetes and spinal pain

Nady Braidy  1 Morteza Izadi  2 Antoni Sureda  3 Nematollah Jonaidi-Jafari  2 Abdolali Banki  4 Seyed F Nabavi  5 Seyed M Nabavi  5

Affiliations expand

·       PMID: 28594115

·       DOI:  10.1002/jcp.26044

Mechanisms of Action Involved in Ozone Therapy: Is healing induced via a mild oxidative stress?

 Sagai M, Bocci V.Med Gas Res. 2011 Dec 20;1:29. doi: 10.1186/2045-9912-1-29.PMID: 22185664     A comprehensive analysis of oxidative stress in the ozone-induced lung inflammation mouse model.Wiegman CH, Li F, Clarke CJ, Jazrawi E, Kirkham P, Barnes PJ, Adcock IM, Chung KF. Clin Sci (Lond). 2014 Mar;126(6):425-40. doi: 10.1042/CS20130039.PMID: 24040961

The usefulness of ozone treatment in spinal pain.Bocci V, Borrelli E, Zanardi I, Travagli V.Drug Des Devel Ther. 2015 May 15;9:2677-85. doi: 10.2147/DDDT.S74518. eCollection 2015.PMID: 26028964 

The Role of Nrf2 in the Antioxidant Cellular Response to Medical Ozone Exposure.Galiè M, Covi V, Tabaracci G, Malatesta M.Int J Mol Sci. 2019 Aug 17;20(16):4009. doi: 10.3390/ijms20164009.PMID: 31426459 

  The effects of ozone therapy and coenzyme Q₁₀ combination on oxidative stress markers in healthy subjects.

Inal M, Dokumacioglu A, Özcelik E, Ucar O.Ir J Med Sci. 2011 Sep;180(3):703-7. doi: 10.1007/s11845-011-0675-7. Epub 2011 Jan 22.PMID: 21258872

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Cited by 10  articles 

Ozone Exposure Induces Metabolic Disorders and NAD+ Depletion Through PARP1 Activation in Spinal Cord Neurons.Ma S, Zhao X, Zhang C, Sun P, Li Y, Lin X, Sun T, Fu Z. Front Med (Lausanne). 2020 Dec 17;7:617321. doi: 10.3389/fmed.2020.617321. eCollection 2020.PMID: 33425964 .

Albiflorin Attenuates Mood Disorders Under Neuropathic Pain State by Suppressing the Hippocampal NLRP3 Inflammasome Activation During Chronic Constriction Injury.Liu P, Chen J, Ma S, Zhang J, Zhou J. Int J Neuropsychopharmacol. 2021 Jan 20;24(1):64-76. doi: 10.1093/ijnp/pyaa076.PMID: 33000169 

  Bioactive Diarylheptanoids from Alpinia coriandriodora.

Cheng XL, Li HX, Chen J, Wu P, Xue JH, Zhou ZY, Xia NH, Wei XY.Nat Prod Bioprospect. 2021 Feb;11(1):63-72. doi: 10.1007/s13659-020-00264-y. Epub 2020 Sep 9.PMID: 32902805

Ozone: a natural bioactive molecule with antioxidant property as potential new strategy in aging and in neurodegenerative disorders.Scassellati C, Galoforo AC, Bonvicini C, Esposito C, Ricevuti G. Ageing Res Rev. 2020 Nov;63:101138. doi: 10.1016/j.arr.2020.101138. Epub 2020 Aug 15.PMID: 32810649 .

 

Decomposition of dimethyl phthalate in an aqueous solution by ozonation with high silica zeolites and UV radiation.

Chen YH, Shang NC, Hsieh DC.J Hazard Mater. 2008 Sep 15;157(2-3):260-8. doi: 10.1016/j.jhazmat.2007.12.091. Epub 2008 Jan 4.PMID: 18280038

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The effect of ozone on hypoxia, hemolysis and morphological change of blood from patients with aortic dissection (AD): a preliminary in vitro experiment of ozonated autohemotherapy for treating AD.

Deng L, Meng W, Li D, Qiu D, Wang S, Liu H.Am J Transl Res. 2018 Jun 15;10(6):1829-1840. eCollection 2018.PMID: 30018723 

 

Complete healing of chronic wounds of a lower leg with haemoglobin spray and regeneration of an accompanying severe dermatoliposclerosis with intermittent normobaric oxygen inhalation (INBOI): a case report.

Barnikol WK, Pötzschke H.Ger Med Sci. 2011 Mar 30;9:Doc08. doi: 10.3205/000131.PMID: 21468328 

 

Regarding: Rosenthal DI, Glatstein E. “We’ve Got a Treatment, but What’s the Disease?” The Oncologist 1996;1.

Lunsford LD, Flickinger JC, Larson D.Oncologist. 1997;2(1):59-61.PMID: 10388030

 

Combination of LC-MS- and GC-MS-based metabolomics to study the effect of ozonated autohemotherapy on human blood.

Ciborowski M, Lipska A, Godzien J, Ferrarini A, Korsak J, Radziwon P, Tomasiak M, Barbas C.J Proteome Res. 2012 Dec 7;11(12):6231-41. doi: 10.1021/pr3008946. Epub 2012 Nov 26.PMID: 23148940

 

Inactivation of Escherichia coli by ozone under bench-scale plug flow and full-scale hydraulic conditions.

Smeets PW, van der Helm AW, Dullemont YJ, Rietveld LC, van Dijk JC, Medema GJ.Water Res. 2006 Oct;40(17):3239-48. doi: 10.1016/j.watres.2006.06.025. Epub 2006 Aug 30.PMID: 16938335


Can the combination of localized “proliferative therapy” with “minor ozonated autohemotherapy” restore the natural healing process?

Gracer RI, Bocci V.Med Hypotheses. 2005;65(4):752-9. doi: 10.1016/j.mehy.2005.04.021.PMID: 15951134

 

The effect of ozone on hypoxia, hemolysis and morphological change of blood from patients with aortic dissection (AD): a preliminary in vitro experiment of ozonated autohemotherapy for treating AD.

Deng L, Meng W, Li D, Qiu D, Wang S, Liu H.Am J Transl Res. 2018 Jun 15;10(6):1829-1840. eCollection 2018.PMID: 30018723 

 

Complete healing of chronic wounds of a lower leg with haemoglobin spray and regeneration of an accompanying severe dermatoliposclerosis with intermittent normobaric oxygen inhalation (INBOI): a case report.

Barnikol WK, Pötzschke H.Ger Med Sci. 2011 Mar 30;9:Doc08. doi: 10.3205/000131.PMID: 21468328   

 

Regarding: Rosenthal DI, Glatstein E. “We’ve Got a Treatment, but What’s the Disease?” The Oncologist 1996;1.

Lunsford LD, Flickinger JC, Larson D.Oncologist. 1997;2(1):59-61.PMID: 10388030

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Kotiaho T, Eberlin MN, Vainiotalo P, Kostiainen R.J Am Soc Mass Spectrom. 2000 Jun;11(6):526-35. doi: 10.1016/S1044-0305(00)00116-1.PMID: 10833026

 

Ozonized autohemotransfusion improves hemorheological parameters and oxygen delivery to tissues in patients with peripheral occlusive arterial disease.

Giunta R, Coppola A, Luongo C, Sammartino A, Guastafierro S, Grassia A, Giunta L, Mascolo L, Tirelli A, Coppola L.Ann Hematol. 2001 Dec;80(12):745-8. doi: 10.1007/s002770100377. Epub 2001 Oct 13.PMID: 11797116 Clinical Trial

 

[The use of the functional state of the joints for the estimation of the effectiveness of the application of oxygen/ozone therapy for the rehabilitative treatment of the patients suffering from knee arthritis].

Baranova IV.Vopr Kurortol Fizioter Lech Fiz Kult. 2018;95(3):42-48. doi: 10.17116/kurort201895342.PMID: 29985380 Clinical Trial. Russian.

 

[A complex medical rehabilitation of patients with coronary heart disease underwent coronary shunting using air-ozone baths].

Shchegol’kov AM, Budko AA, Sychev VV, Azarova EK, Arseniĭ TV.Voen Med Zh. 2009 Aug;330(8):30-2.PMID: 19916311 Clinical Trial. Russian.

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Natural killer cell activity unaffected by ozonated autohemotherapy in patients with end-stage renal disease on maintenance renal replacement therapy.

Biedunkiewicz B, Tylicki L, Rachon D, Hak L, Nieweglowski T, Chamienia A, Debska-Slizien A, Mysliwska J, Rutkowski B.Int J Artif Organs. 2004 Sep;27(9):766-71. doi: 10.1177/039139880402700906.PMID: 15521216 Clinical Trial

 

Efficacy and tolerability of medical ozone gas insufflations in patients with osteonecrosis of the jaw treated with bisphosphonates-Preliminary data: Medical ozone gas insufflation in treating ONJ lesions.

Ripamonti CI, Maniezzo M, Boldini S, Pessi MA, Mariani L, Cislaghi E.J Bone Oncol. 2012 Sep 24;1(3):81-7. doi: 10.1016/j.jbo.2012.08.001. eCollection 2012 Dec.PMID: 26909261 

 

 

The case for oxygen-ozonetherapy.

Bocci V.Br J Biomed Sci. 2007;64(1):44-9. doi: 10.1080/09674845.2007.11732755.PMID: 17444419 Review

 

 

Ozone Gas Bath Combined with Endovenous Laser Therapy for Lower Limb Venous Ulcers: A Randomized Clinical Trial.

Zhou YT, Zhao XD, Jiang JW, Li XS, Wu ZH.J Invest Surg. 2016 Oct;29(5):254-9. doi: 10.3109/08941939.2016.1149637. Epub 2016 Mar 24.PMID: 27010682 Clinical Trial.

 

 

May oxygen-ozone therapy improves cardiovascular disorders?

Bocci V, Travagli V, Zanardi I.Cardiovasc Hematol Disord Drug Targets. 2009 Jun;9(2):78-85. doi: 10.2174/187152909788488681.PMID: 19519366 Review.

 

Extracorporeal blood oxygenation and ozonation (EBOO) in man. preliminary report.

Di Paolo N, Bocci V, Garosi G, Borrelli E, Bravi A, Bruci A, Aldinucci C, Capotondo L.Int J Artif Organs. 2000 Feb;23(2):131-41.PMID: 10741810

 

Intravesical ozone therapy for progressive radiation-induced hematuria.

Clavo B, Gutiérrez D, Martín D, Suárez G, Hernández MA, Robaina F.J Altern Complement Med. 2005 Jun;11(3):539-41. doi: 10.1089/acm.2005.11.539.PMID: 15992242

 

Anti-fungal potential of ozone against some dermatophytes.

Ouf SA, Moussa TA, Abd-Elmegeed AM, Eltahlawy SR.Braz J Microbiol. 2016 Jul-Sep;47(3):697-702. doi: 10.1016/j.bjm.2016.04.014. Epub 2016 Apr 21.PMID: 27287337